Coding

Part:BBa_K2958006:Design

Designed by: Angelica Sabandal   Group: iGEM19_ULaVerne_Collab   (2019-07-24)


Single Chain Proinsulin (Native Insulin with Linker)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted the linker used to result in a PI of 5.50 in order to match the PI of native insulin. PI was checked on serial cloner. This sequence is codon optimized for E. coli K12.


Source

This part comes from a genomic sequence made on IDT.

References